General Science/Basic Science botany Biotechnology Sample Test,Sample questions

Question:
A branch of botany is

1. Genetic engineering

2. Plant Tissue culture

3. Plant Physiology

4. Plant ecology


Question:
An example of a fusogenic agent is


1.Methanol

2. Ethyl alcohol

3. Polyethelene alcohol

4. Polyethelene glycol (PEG)


Question:
Bacillus subtilis and streptomyces spp. are Prokaryotes which are used as hosts for introducing




1.Plasmid

2.Replicon

3.Auxin

4.rDNA


Question:
Cells of higher plants are grown artificially in laboratory conditions using the technique of

1. Molecular cloning

2. Gene cloning

3. Tissue culture

4. Hybridzation


Question:
Cellulase and Cellulysin are the cell wall degrading enzyme mixtures which can digest

1. Cell wall

2. Epidermal cells

3. Mesophyll

4. Xylem vessels


Question:
DNA ligase was discovered in

1.1906

2.1910

3.1966

4.1970


Question:
DNA of donor is cut into pieces using

1. Vector

2. Plasmid

3. Endonuclease

4. Inoculum


Question:
DNA recombinant technology is also known as




1.Gene manipulation

2.Totipotency

3.Splicing

4.Gene cloning


Question:
Each colony is a collection of bacteria having many copies of rDNA fragments called



1. Molecular cloning

2.Splicing

3.Cloning vehicles

4.Vectors


Question:
Flamed and cooled platinum wire is used to transfer





1.Bacteria

2.Fungi

3.Callus

4.Inoculum


Question:
Foot and mouth disease of cattle is cured by


1. Insulin

2.Cytokinin

3. Vaccine

4.Interferon


Question:
Genes are directly introduced into the eukaryotie host for cloning through a


1.Plasmid

2. Vector

3. Endonuclease

4. Hormone


Question:
Genetic engineering started with flying colours in the early

1.1900

2.1950

3.1960

4.1970


Question:
If the DNA fragments of donor do not replicate in the host they are attached to a suitable


1. DNA ligase

2.Plasmid

3.Splicing

4.Replicon


Question:
Industrial production of enzymes vitamins vaccines and bio-fertilizers are the products of




1.Ploidy

2.Genetics

3.Physiology

4.Biotechnology


Question:
Inoculated culture vessels are protected from contamination of other organism by


1.Cleaning

2. Cotton plug

3. Oil cloth

4. Cork plug


Question:
Inter specific crosses in industrial micro-organisms are made easier by


1. Protoplast fusion

2. Tissue culture

3. Genetic engineering

4.Totipotency


Question:
Introducing the nitrogen fixing genes from bacteria to major food crops is possible through

1. Tissue culture

2.Hybridization

3. Protoplast

4. Genetic engineering


Question:
Joining of donor DNA fragments and vector DNA fragments with the help of DNA ligase enzyme is known as



1. Gene cloning

2.Splicing

3.Gene manipulation

4.Molecular cloning


Question:
Molecular cloning is also known as




1. Splicing

2.Gene cloning

3.Gene manipulation

4.Hardening


Question:
Plants obtained through tissue culture method constitute genetically uniform population called



1. Clone

2. Callus

3.Inoculum

4.Hardening


Question:
Protoplasmic fusion is of great importance in plants which are incompatible to

1. Molecular cloning

2. Gene cloning

3.Hybridization

4. Tissue culture


Question:
Replicons are also known as





1.Ligase

2.Plasmids

3.Auxin

4.Vectors


Question:
Restriction endonucleases are discovered in

1.1910

2.1906

3.1966

4.1970


Question:
Several plantlets are developed from the




1. Clone

2.Inoculum

3.Callus

4.Plasmid


Question:
Somatic hybridization is brought about by employing the technique of

1.Tissue culture

2.Splicing

3.Totipotency

4.Hardening


Question:
Suitable host in genetic engineering to introduce DNA fragments of donor is


1.Yeast

2. Bacillus subtilis

3. Escherichia coli

4. Bacteriophages


Question:
The ability of every living plant cell to produce the entire plant is called

1. Plasmid

2. Protoplast

3. Gene cloning

4. Totipotency


Question:
The agent which promotes the fusion of protoplasts is called

1. Mutagenic agent

2. Fusogenic agent

3. Chemical agent

4. Cellulase


Question:
The antiviral protein produced by virus infected cells is called

1.Insulin

2. Auxin

3. Vaccine

4. Interferon


Question:
The cells in tissue culture divide and form a mass of undifferentiated tissue called


1.Inoculum

2. Plasmid

3. Replicon

4. Callus


Question:
The contaminated microorganism in culture medium is




1.Fungi

2.Algae

3.Callus

4.Cytokinin


Question:
The culture medium is prepared from


1. Proteins

2.Vitamins

3. Agar

4.Bacteria


Question:
The DNA of vector is cut into fragments by

1.plasmid

2. Auxin

3. Restriction endonucleases

4. Cytokinin


Question:
The donor DNA fragments and vector DNA fragments are joined together by





1.DNA ligase

2.Plasmid

3.Auxin

4.Cytokinin


Question:
The enzyme which acts like a paste molecule to join DNA fragments




1.Plasmid

2.Clone

3. DNA ligase

4.Protease


Question:
The extra-chromosomal circular DNA found in the E.coli is



1.Plasmid

2. DNA ligase

3.Vector

4. Plasmid


Question:
The first step in protoplasmic fusion is the

1. Isolation of protoplasts

2. Fusion of protoplasts

3. Growth protoplasts

4. Regeneration of cell wall


Question:
The following is not a branch of biotechnology

1.Genetic engineering

2.Tissue culture

3.Physiology

4.Protoplast fusion


Question:
The following is used in industrial fermentation to produce food and drinks




1.Yeast

2.Algae

3.Vitamins

4.Vaccines


Question:
The following ratio of hormonal concentration is required to induce callus to form shoot and root


1.Gibberellins to auxin

2. Auxin to cytokinin

3. Gibberellins to cytokinin

4. Ethylene to auxins


Question:
The host bacterium follows the instruction of the





2

1.DNA ligase

2.Foreign rDNA

3.Plasmid

4.Auxin


Question:
The inherent capacity of an isolated plant cell to give rise to a whole plant is called

1. Totipotency

2. Protoplast

3. Hybridization

4. Gene manipulation


Question:
The instrument used for keeping inoculated vessels at a desired constant temperature is


1. Platinum wire

2.Micropipette

3. Petriplate

4. Incubator


Question:
The instrument used to transfer inoculums under aseptic condition is


1.Incubator

2. Culture tube

3. Micropipette

4. Petriplate


Question:
The interspecfic somatic hybrids of tobacco and petunia are produced by the technique of

1. Tissue culture

2. Protoplasmic fusion

3. Genetic engineering

4. Gene cloning


Question:
The isolated protoplasts are grown in

1.Hypotonic culture medium

2. Hypertonic culture medium

3. Isotonic culture medium

4. Agar culture medium


Question:
The isolated protoplasts are made to fuse to produce a hybrid cell in

1. Tissue culture

2. Genetic engineering

3.Protoplast fusion

4. Gene cloning


Question:
The mechanism which has the ability to engineer new organisms is known as





1.Totipotency

2.Molecular cloning

3.Genetic engineering

4.Splicing


Question:
The molecular scissors which cut DNA at specific sites are

1. Plasmids

2. Fusogenic agents

3.Inoculum

4. Restriction enzymes


Question:
The most suitable vectors are





1.Yeast

2.Bacteriophages

3.Moulds

4.Algae


Question:
The other name for hybrid DNA is





1.Recombinant DNA (rDNA)

2. DNA ligase

3.Plasmid

4.Replicon


Question:
The plant cell walls are digested by specific cell wall degrading

1. Protein mixtures

2. Vitamin mixtures

3.Enzyme mixtures

4. Hormonal mixtures


Question:
The process of transferring plantlets to pots and subjected gradually to natural environmental conditions is called




1. Hardening

2.Totipotency

3.Cloning

4.Splicing


Question:
The production of insulin interferon human growth hormones proteins and vaccines are based on

1. Tissue culture

2. Protoplast fusion

3. Genetic engineering

4. Hybridization


Question:
The production of new characteristics by introducing new genes and altering the genome is known as



1.DNA recombinant technology

2. Protoplast fusion

3.Totipotency

4.Splicing


Question:
The rapid and cheaper method of propagating plants with desire qualities is

1. Molecular cloning

2. Gene cloning

3. Tissue cloning

4. Genetic cloning


Question:
The rDNA is introduced into this host




1. E.coli

2.Yeast

3.Mould

4.Algae


Question:
The recent and fascinating branch of biology is


1. Physiology

2.Genetics

3. Genetic engineering

4.Ecology


Question:
The rigid plant cell wall is digested by



1.Pectinase

2. Protease

3. Amylase

4.Sucrase


Question:
The technique used industrially to multiply plants which are difficult to propagate by conventional means is

1. Protoplast fusion

2. Tissue culture

3.Hybridization

4. Genetic engineering


Question:
The technique which is useful in genetic engineering to introduce rDNA and other DNA fragments into higher plant cells in culture is

1. Protoplasmic fusion

2. Tissue culture

3. Hardening

4.Splicing


Question:
The transfer of inoculums is done under

1. Aseptic condition

2. Septic condition

3. Hygenic condition

4. Environmental condition


Question:
The two different genetic sources protoplasts usually used are



1.Mesophyll and parenchyma cells

2.Mesophyll and epidermal cells

3. Epidermal cells and parenchyma cells

4.Hypodermal and mesophyll cells


Question:
Tissue culture technique is successfully employed to bring about

1. Protoplasmic fusion

2. Gene manipulation

3. Splicing

4. Hardening


Question:
Vectors are also known as

1.DNA ligase

2.Plasmid

3. Cloning vehicles

4. Inoculum


Question:
Very small number of isolated living cells is called

1.Plasmid

2. Vector

3. Auxin

4. Inoculum


Question:
Virus free potatoes tulips sugarcanes and orchids are obtained by the technique of


1.Hardening

2. Totipotency

3. Tissue culture

4. Splicing


Question:
Yoghurt is a food product produced by employing




1.Hybridization

2.Tissue culture

3.Protoplast fusion

4.Biotechnology


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