In genetic engineering, it is necessary to cut DNA out of bacteria and eukaryotes. To insert the human insulin gene into a bacteria, one should use

Question:In genetic engineering, it is necessary to cut DNA out of bacteria and eukaryotes. To insert the human insulin gene into a bacteria, one should use

1.two different restriction enzymes so that the pieces would not reanneal

2.the same restriction enzyme so that both pieces will have the same sticky ends

3.methylated bacterial DNA so that only some of it will be spliced

4.two different ligases to reanneal the DNA


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