What would happen if serum is omitted from the ELISA, but all other steps remain same?

Question:What would happen if serum is omitted from the ELISA, but all other steps remain same?

1. Anti-human Ig-conjugate would not bind and be washed away

2.The OD values would be nearly the same as the assay control

3.Both (a) and (b)

4.Anti-human Ig-conjugate would bind non-specifically to the ELISA plate


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