In lysozyme catalysis, which of the following does not contribute?

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Question: In lysozyme catalysis, which of the following does not contribute?

1.The abnormally high pKa of Glu35

2.The strained conformation of the D sugar

3.Formation of a covalent intermediate at Asp52

4.Formation of a covalent intermediate at Ser195

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  1. In lysozyme catalysis, which of the following does not contribute?
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