Why do fluorescence spectrometers often use double-beam optics?

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Question:Why do fluorescence spectrometers often use double-beam optics?

1.So a reference solution can be used

2.To compensate for beam attenuation by the monochromator

3.To compensate for power fluctuations in the radiation source

4.All of the above

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  1. Why do fluorescence spectrometers often use double-beam optics?
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