Void volume refers to the

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Question:Void volume refers to the

1. total volume of eluent in the column the remainder being taken up by the packing material

2.the volume of solvent contained in a liquid chromatographic column

3.the time required for the gradient to reach the column

4.the volume of the column between the point at which solvents are mixed and the beginning of the column

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  1. Void volume refers to the
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