Which of the following is used to determine the appropriate stringency (Wallace rule)?

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Question:Which of the following is used to determine the appropriate stringency (Wallace rule)?

1.Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

2.Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)

3. Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

4. Tm = 69.3° + 0.41 (%)G+C -650/1

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  1. Which of the following is used to determine the appropriate stringency (Wallace rule)?
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